ISO 6579-1:2017(EN) Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp.

ISO 6579-1:2017 describes a horizontal method for the detection of Salmonella spp. in food (including milk and milk products, originally described in ISO 6785), in animal feed, in animal faeces, and in environmental samples from the primary production stage (the latter two were originally described in ISO 6579:2002)
The main changes, listed in the foreword, introduced in ISO 6579-1:2017 compared to ISO 6579:2002, are considered as minor.
A procedure for the enumeration of Salmonella spp. is described in ISO/TS 6579-2
Guidance for serotyping of Salmonella spp. is described in ISO/TR 6579-3.

ISO 6579-1:2017 specifies a horizontal method for the detection of Salmonella. It is applicable to the following:
— Products intended for human consumption and the feeding of animals;
— Environmental samples in the area of food production and food handling;
— Samples from the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi,
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.
ISO/TS 6579-2:2012(EN) Microbiology of food and animal feed — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 2: Enumeration by a miniaturized most probable number technique
ISO 6579-1:2017 gives a method for the enumeration of Salmonella spp. present in:
— Products intended for human consumption and for the feeding of animals;
— Environmental samples in the area of food production and food handling;
— Animal faeces;
— Environmental samples from the primary production stage;
by calculation of the most probable number (MPN).
The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport–Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae.
It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi.
The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).
ISO/TR 6579-3:2014(EN) Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 3: Guidelines for serotyping of Salmonella spp.
ISO/TR 6579-3:2014 gives guidance on the procedure for serotyping Salmonella serovars and is applicable to the serotyping of pure cultures of Salmonella spp., independent of the source from which they are isolated.
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